Anti-Bak抗体[AT38E2] (ab104124)
Key features and details
- Mouse monoclonal [AT38E2] to Bak
- Suitable for: Flow Cyt, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Bak抗体[AT38E2]
参阅全部 Bak 一抗 -
描述
小鼠单克隆抗体[AT38E2] to Bak -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to amino acids 29-187 of Human Bak, purified from E. coli.
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阳性对照
- WB: Mouse lung lysate, 293T, HeLa, A431, A549, Jurkat, MCF7, and PC3 cell lysates ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AT38E2 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. -
组织特异性
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
细胞定位
Mitochondrion membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 578 Human
- Entrez Gene: 12018 Mouse
- Omim: 600516 Human
- SwissProt: Q16611 Human
- SwissProt: O08734 Mouse
- Unigene: 485139 Human
- Unigene: 2443 Mouse
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别名
- Apoptosis regulator BAK antibody
- BAK antibody
- BAK like antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAK knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab104124 observed at 0 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab104124 was found to be non-specific when BAK knockout samples were used. Wild-type and BAK knockout samples were subjected to SDS-PAGE. Ab104124 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image was produced using the ascites version of this antibody.
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Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ab104124 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
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Immunocytochemistry/ Immunofluorescence analysis of BAK in HeLa cells. The cell was stained with ab104124 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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All lanes : Anti-Bak antibody [AT38E2] (ab104124) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 23 kDa -
Anti-Bak antibody [AT38E2] (ab104124) at 1/500 dilution + Mouse lung lyasate at 35 µg
Secondary
Goat anti-mouse HRP conjugate
Developed using the ECL technique.
Predicted band size: 23 kDaThis image was produced using the ascites version of this antibody.
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Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).
This image was produced using the ascites version of this antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab104124 被引用在 5 文献中.
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Loo LSW et al. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11:378 (2020). PubMed: 32424151
- Singh A et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). PubMed: 28289414
- Liu P et al. Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. Iran J Basic Med Sci 20:1260-1267 (2017). PubMed: 29299204
- Mebratu YA et al. Bik reduces hyperplastic cells by increasing Bak and activating DAPk1 to juxtapose ER and mitochondria. Nat Commun 8:803 (2017). PubMed: 28986568