Anti-Bak抗体- Aminoterminal end (ab62486)

概述

  • 产品名称Anti-Bak抗体- Aminoterminal end
    参阅全部 Bak 一抗
  • 描述
    兔多克隆抗体to Bak - Aminoterminal end
  • 特异性This antibody reacts with Bak
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 13 amino acid synthetic peptide from near the amino terminus of human Bak

  • 阳性对照
    • L1210 cell lysates and L1210 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ion Exchange Chromatography
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab62486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
ICC Use a concentration of 2 µg/ml.

靶标

  • 功能In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
  • 组织特异性Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 细胞定位Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator BAK antibody
    • BAK antibody
    • BAK like antibody
    • Bak NT antibody
    • BAK_HUMAN antibody
    • Bak1 antibody
    • Bcl 2 homologous antagonist/killer antibody
    • Bcl 2 like 7 protein antibody
    • Bcl-2 homologous antagonist/killer antibody
    • Bcl-2-like protein 7 antibody
    • BCL2 antagonist/killer 1 antibody
    • Bcl2 like 7 Protein antibody
    • Bcl2-L-7 antibody
    • BCL2L7 antibody
    • CDN1 antibody
    • Cell death inhibitor 1 antibody
    • MGC117255 antibody
    • MGC3887 antibody
    • NBak antibody
    • Pro apoptotic protein BAK antibody
    see all

Anti-Bak antibody - Aminoterminal end 图像

  • Lane 1 : Anti-Bak antibody - Aminoterminal end (ab62486) at 1 µg/ml
    Lane 2 : Anti-Bak antibody - Aminoterminal end (ab62486) at 2 µg/ml

    Lane 1 : L1210 cell lysates
    Lane 2 : L1210 cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit IgG secondary antibody

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of Bak in L1210 cells with ab62486 antibody at 2 µg/ml.

Anti-Bak antibody - Aminoterminal end (ab62486)参考文献

ab62486 has not yet been referenced specifically in any publications.

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