概述

  • 产品名称Anti-Bag5抗体
    参阅全部 Bag5 一抗
  • 描述
    小鼠多克隆抗体to Bag5
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human Bag5 (NP_004864.1).

  • 阳性对照
    • WB: Human spleen tissue lysate; HeLa cell lysate; Bag5 transfected 293T cell lysate ICc/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
  • 序列相似性Contains 5 BAG domains.
  • 结构域The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAG family molecular chaperone regulator 5 antibody
    • BAG-5 antibody
    • BAG5 antibody
    • BAG5_HUMAN antibody
    • Bcl 2 associated athanogene 5 antibody
    • Bcl-2-associated athanogene 5 antibody
    • BCL2 associated athanogene 5 antibody
    • KIAA0873 antibody
    see all

Anti-Bag5 antibody 图像

  • Anti-Bag5 antibody (ab88100) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • Anti-Bag5 antibody (ab88100) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-Bag5 antibody (ab88100) at 1 µg/ml

    Lane 1 : Bag5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 51 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab88100, at 10 µg/ml, staining Bag5 in HeLa cell by Immunofluorescence.

Anti-Bag5 antibody (ab88100)参考文献

ab88100 has not yet been referenced specifically in any publications.

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