概述

  • 产品名称Anti-Bag3抗体
    参阅全部 Bag3 一抗
  • 描述
    兔多克隆抗体to Bag3
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Bag3.

    (Peptide available as ab96631.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human lung; Human heart; Human spleen;

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab86298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • 疾病相关Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • 序列相似性Contains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAG 3 antibody
    • BAG family molecular chaperone regulator 3 antibody
    • BAG-3 antibody
    • Bag3 antibody
    • BAG3_HUMAN antibody
    • Bcl 2 binding protein antibody
    • Bcl-2-associated athanogene 3 antibody
    • Bcl-2-binding protein Bis antibody
    • BCL2 associated athanogene 3 antibody
    • BCL2 binding athanogene 3 antibody
    • BIS antibody
    • CAIR 1 antibody
    • Docking protein CAIR 1 antibody
    • Docking protein CAIR-1 antibody
    • MFM6 antibody
    see all

Anti-Bag3 antibody 图像

  • All lanes : Anti-Bag3 antibody (ab86298) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ICC/IF image of ab86298 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86298 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 cells in PFA at 5ug/ml, and in HeLa, Hek293, HepG2, MCF-7 cells in Methanol at 5ug/ml.

  • IHC image of Ab86298 staining in Humna Cervical Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab86298, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Bag3 antibody (ab86298)参考文献

ab86298 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Mouse Cell (Primary Hepatocytes)
Specification Primary Hepatocytes
Permeabilization No
Fixative Paraformaldehyde
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提交于 Jan 23 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification primary hepatoctyes
Blocking step I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
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提交于 Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"