概述

  • 产品名称Anti-BAG2抗体
    参阅全部 BAG2 一抗
  • 描述
    兔多克隆抗体to BAG2
  • 经测试应用适用于: WB, IHC-P, IHC-Fr, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • Lymphoid, and many cancer cell lines. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab47106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP 1/50 - 1/200.
ICC/IF 1/1000 - 1/5000.

靶标

  • 功能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • 序列相似性Contains 1 BAG domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAG 2 antibody
    • BAG family molecular chaperone regulator 2 antibody
    • BAG-2 antibody
    • BAG-family molecular chaperone regulator-2 antibody
    • Bag2 antibody
    • BAG2_HUMAN antibody
    • BCL 2 associated athanogene 2 antibody
    • Bcl-2-associated athanogene 2 antibody
    • BCL2 associated athanogene 2 antibody
    • BCL2-associated athanogene 2 antibody
    • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
    • dJ417I1.2 antibody
    • KIAA0576 antibody
    • MGC149462 antibody
    • OTTHUMP00000016668 antibody
    see all

Anti-BAG2 antibody 图像

  • Anti-BAG2 antibody (ab47106) at 1/2000 dilution + HeLa cells

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Immunofluorescence confocal microscopy of BAG-2 using ab47106 at 1:2000 on HeLa cells growing on coverslips left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.

Anti-BAG2 antibody (ab47106)参考文献

This product has been referenced in:
  • Horikawa I  et al. Autophagic degradation of the inhibitory p53 isoform ?133p53a as a regulatory mechanism for p53-mediated senescence. Nat Commun 5:4706 (2014). Read more (PubMed: 25144556) »
  • Rogon C  et al. HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis. Mol Biol Cell 25:2260-71 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24899640) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us with your question. We only have the full size vials of each of our products, however we do guarantee that all antibodies will work in tested species and applications as stated on the datasheet for up to 6 months. So, i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hek293T)
Loading amount 30 µg
Specification Hek293T
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Mirjam Eckert

Verified customer

提交于 Oct 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 40 µg
Specification Hela
Treatment lysed by RIPA w/o SDS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2%
Username

Mr. Jörg Hoehfeld

Verified customer

提交于 Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"