Bag1 overexpression 293T裂解液(全细胞) (ab94217)

概述

  • 产品名称Bag1 overexpression 293T裂解液(全细胞)
  • 描述
    Bag1 overexpression裂解液
  • 常规说明ab94217 is a 293T cell transfected lysate in which Human Bag1 has been transiently over-expressed using a pCMV-Bag1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Tissue specificity: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). Similarity: Contains 1 BAG domain. Contains 1 ubiquitin-like domain. PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

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应用

Our Abpromise guarantee covers the use of ab94217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Bag1 overexpression 293T lysate (whole cell) 图像

  • ab94217 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Bag1 antibody (ab54817) at 1/500 dilution

    Lane 1 : Bag1 293T Transfected Lysate - Positive Control (ab94217)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

Bag1 overexpression 293T lysate (whole cell) (ab94217)参考文献

ab94217 has not yet been referenced specifically in any publications.

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