概述

  • 产品名称
    Anti-Bag1抗体[Y166]
    参阅全部 Bag1 一抗
  • 描述
    兔单克隆抗体[Y166] to Bag1
  • 特异性
    The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues following the repeat 7 of human Bag-1.

  • 阳性对照
    • HeLa cell lysate and cells, human prostate carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/80.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
    • 组织特异性
      Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
    • 序列相似性
      Contains 1 BAG domain.
      Contains 1 ubiquitin-like domain.
    • 翻译后修饰
      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • 细胞定位
      Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Bag 1 antibody
      • BAG family molecular chaperone regulator 1 antibody
      • BAG-1 antibody
      • BAG1 antibody
      • BAG1_HUMAN antibody
      • BCL 2 Associated Athanogene 1 antibody
      • BCL 2 associated athanogene antibody
      • BCL 2 binding athanogene 1 antibody
      • Bcl-2-associated athanogene 1 antibody
      • BCL2 associated athanogene 1 antibody
      • BCL2 associated athanogene antibody
      • Glucocorticoid receptor-associated protein RAP46 antibody
      • HAP1 antibody
      • Haptoglobin antibody
      • RAP 46 antibody
      • RAP46 antibody
      see all

    Anti-Bag1 antibody [Y166] 图像

    • Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 31 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 46 kDa (possible isoform),50 kDa (possible isoform).
    • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.

    Anti-Bag1 antibody [Y166] (ab32109)参考文献

    This product has been referenced in:
    • Mashukova A  et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci 127:3568-77 (2014). Read more (PubMed: 24876225) »
    • Anderson LR  et al. BAG-1 overexpression attenuates luminal apoptosis in MCF-10A mammary epithelial cells through enhanced RAF-1 activation. Oncogene 29:527-38 (2010). ICC/IF ; Human . Read more (PubMed: 19881545) »

    See all 2 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    Human Cell (Human primary fibroblasts)
    Specification
    Human primary fibroblasts
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    20 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    200 µg
    Specification
    HeLa cells
    Immuno-precipitation step
    Protein A/G
    Username

    Abcam user community

    Verified customer

    提交于 Apr 06 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Svetlana Khoronenkova

    Verified customer

    提交于 Aug 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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