Anti-Bag1抗体- Aminoterminal end (ab45411)

概述

  • 产品名称Anti-Bag1抗体- Aminoterminal end
    参阅全部 Bag1 一抗
  • 描述
    兔多克隆抗体to Bag1 - Aminoterminal end
  • 特异性This antibody is specific for Bag1.
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 14 amino acid peptide from near the amino terminus of human Bag 1.

  • 阳性对照
    • PC-3 cell lysate.

应用

Our Abpromise guarantee covers the use of ab45411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

靶标

  • 功能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
  • 组织特异性Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
  • 序列相似性Contains 1 BAG domain.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bag 1 antibody
    • BAG family molecular chaperone regulator 1 antibody
    • BAG-1 antibody
    • BAG1 antibody
    • BAG1_HUMAN antibody
    • BCL 2 Associated Athanogene 1 antibody
    • BCL 2 associated athanogene antibody
    • BCL 2 binding athanogene 1 antibody
    • Bcl-2-associated athanogene 1 antibody
    • BCL2 associated athanogene 1 antibody
    • BCL2 associated athanogene antibody
    • Glucocorticoid receptor-associated protein RAP46 antibody
    • HAP1 antibody
    • Haptoglobin antibody
    • RAP 46 antibody
    • RAP46 antibody
    see all

Anti-Bag1 antibody - Aminoterminal end 图像

  • Lane 1 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 1 µg/ml
    Lane 2 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 2 µg/ml

    Lane 1 : PC-3 cell lysate
    Lane 2 : PC-3 cell lysate


    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab45411 at 2µg/ml staining Bag1-Aminoterminal end in human lymph nodes by IHC

  • Immunofluorescence of BAG-1 in Human Lymph Node cells using ab45411 at 20 ug/ml.

Anti-Bag1 antibody - Aminoterminal end (ab45411)参考文献

ab45411 has not yet been referenced specifically in any publications.

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