概述

  • 产品名称Anti-Bag1抗体
    参阅全部 Bag1 一抗
  • 描述
    兔多克隆抗体to Bag1
  • 经测试应用适用于: WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to a sequence at the C-terminal of Human Bag1.

性能

应用

Our Abpromise guarantee covers the use of ab112493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. The detection limit for Bag1 is approximately 2.5ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

靶标

  • 功能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
  • 组织特异性Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
  • 序列相似性Contains 1 BAG domain.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bag 1 antibody
    • BAG family molecular chaperone regulator 1 antibody
    • BAG-1 antibody
    • BAG1 antibody
    • BAG1_HUMAN antibody
    • BCL 2 Associated Athanogene 1 antibody
    • BCL 2 associated athanogene antibody
    • BCL 2 binding athanogene 1 antibody
    • Bcl-2-associated athanogene 1 antibody
    • BCL2 associated athanogene 1 antibody
    • BCL2 associated athanogene antibody
    • Glucocorticoid receptor-associated protein RAP46 antibody
    • HAP1 antibody
    • Haptoglobin antibody
    • RAP 46 antibody
    • RAP46 antibody
    see all

Anti-Bag1 antibody 图像

  • All lanes : Anti-Bag1 antibody (ab112493)

    Lane 1 : Recombinant Human BAG-1 Protein at 0.01 µg
    Lane 2 : Recombinant Human BAG-1 Protein at 0.005 µg
    Lane 3 : Recombinant Human BAG-1 Protein at 0.0025 µg
    Lane 4 : MCF-7 Whole Cell Lysate


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab112493 staining Bag1 in Rat intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection by ABC/DAB staining.

Anti-Bag1 antibody (ab112493)参考文献

ab112493 has not yet been referenced specifically in any publications.

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