Anti-BAFF抗体[Buffy 2] (FITC) (ab16082)

概述

  • 产品名称Anti-BAFF抗体[Buffy 2] (FITC)
    参阅全部 BAFF 一抗
  • 描述
    大鼠单克隆抗体[Buffy 2] to BAFF (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant soluble protein, corresponding to amino acids 83 - 285 of Human BAFF.

  • 阳性对照
    • Ramos cells, HL60 AML cells
  • 常规说明1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab16082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
Flow Cyt
  • 应用说明IHC-P: 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use streptavidin-biotin amplification (see recommended protocol).
    Flow Cyt: Use at a concentration of 1 µg/ml.
    Incubate cells on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of Buffy-2-FITC antibody (testing conditions for HEK 293T cells (5 x 105) mock transfected or transfected with an expression plasmid enabling surface expression of full length hBAFF).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    • 组织特异性Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
    • 序列相似性Belongs to the tumor necrosis factor family.
    • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
      N-glycosylated.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ApoL related ligand TALL 1 antibody
      • B cell Activating Factor antibody
      • B lymphocyte stimulator antibody
      • B-cell-activating factor antibody
      • BAFF antibody
      • BLyS antibody
      • CD 257 antibody
      • CD257 antibody
      • CD257 antigen antibody
      • Delta BAFF antibody
      • Dendritic cell derived TNF like molecule antibody
      • Dendritic cell-derived TNF-like molecule antibody
      • DTL antibody
      • DTL precursor antibody
      • PRO738 antibody
      • soluble form antibody
      • TALL 1 antibody
      • TALL-1 antibody
      • TALL1 antibody
      • THANK antibody
      • TN13B_HUMAN antibody
      • TNF and APOL related leukocyte expressed ligand 1 antibody
      • TNF homolog that activates apoptosis antibody
      • TNF homolog that activates apoptosis NKFB and JNK. antibody
      • TNF- and APOL-related leukocyte expressed ligand 1 antibody
      • TNFSF13B antibody
      • TNFSF20 antibody
      • Tumor necrosis factor (ligand) superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 20 antibody
      • Tumor necrosis factor like protein ZTNF4 antibody
      • Tumor necrosis factor superfamily member 13B antibody
      • UNQ401 antibody
      • ZTNF4 antibody
      see all

    Anti-BAFF antibody [Buffy 2] (FITC) 图像

    • Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows  indicate vessels staining positive for BAFF using ab16082.
      Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

    • Ab16082 detects membrane-bound human BAFF by FACS.
      Method: HEK 293T cells (5 x 10e5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of ab16082. After washing, cells were analyzed by flow cytometry.

    Anti-BAFF antibody [Buffy 2] (FITC) (ab16082)参考文献

    This product has been referenced in:
    • Huard B  et al. BAFF production by antigen-presenting cells provides T cell co-stimulation. Int Immunol 16:467-75 (2004). Read more (PubMed: 14978020) »
    • Novak AJ  et al. Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome. Blood 104:2247-53 (2004). Read more (PubMed: 15251985) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry.

    This antibody was raised against soluble Buffy2, so I believe it detects intracellular Buffy2. Below I have included a link to a general intracellular staining protocol. Below that is the recommended FACS protocol ...

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    This antibody (ab16082) recognizes the extracellular domain of BAFF, and is guaranteed for flow cytometry. We do not currently have any products that recognize the intracellular domain of BAFF.

    Le laboratoire m'a confirmé que de récents tests ont montré que l'anticorps fonctionne en effet en cytométrie en flux pour le marquage extracellulaire et intracellulaire également.

    La fiche technique de l'ant...

    Read More

    Merci de nous avoir contactés.

    Il a été décrit que le clone [Buffy 2] ne distingue pas les 2 formes de la BAFF et peut donc reconnaître à la fois la forme soluble et la forme fixée à la me...

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    Thank you for your enquiry. This antibody is supply in a 50µl volume. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    I'm very sorry for the delay in finding out the protocol for FACS analysis; I have just received the following information from the source of ab16082: Method: HEK 293T cells (5 x 10e5) were mock transfected (thin line) or transfected with an express...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"