概述
- 产品名称Anti-BAFF抗体
参阅全部 BAFF 一抗 - 描述兔多克隆抗体to BAFF
- 经测试应用适用于: Sandwich ELISA, WBmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human BAFF.
(Peptide available as ab69714.)
- 阳性对照
- 购买相配的WB阳性对照Recombinant Human BAFF protein
- This antibody gave a positive signal in the following Human Whole Cell Lysates: Jurkat, HL60, MOLT4
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
- sELISA pair antibody
应用
Our Abpromise guarantee covers the use of ab65360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to BAFF (ab9359). For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab9359 as Capture. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
靶标
- 功能Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
- 组织特异性Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
- 序列相似性Belongs to the tumor necrosis factor family.
- 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. - 细胞定位Secreted and Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 504507 Cow
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Entrez Gene: 100038026 Pig
- Entrez Gene: 100101577 Rabbit
- Entrez Gene: 498666 Rat
- Entrez Gene: 780517 Sheep
- Omim: 603969 Human
see all -
别名
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
Anti-BAFF antibody 图像
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Sandwich ELISA - BAFF antibody (ab65360)Standard Curve for BAFF (Analyte: ab79745) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to BAFF (ab9359) at 1ug/ml and Detector Antibody Rabbit polyclonal to BAFF (ab65360) at 0.1ug/ml -
Western blot - Anti-BAFF antibody (ab65360)All lanes : Anti-BAFF antibody (ab65360) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Exposure time : 90 seconds
实验方案
Anti-BAFF antibody (ab65360)参考文献
ab65360 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"