Anti-BAF57/SMARCE1抗体(ab84420)

概述

  • 产品名称Anti-BAF57/SMARCE1抗体
    参阅全部 BAF57/SMARCE1 一抗
  • 描述
    兔多克隆抗体to BAF57/SMARCE1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide designed within residues: G TSTPEDKE SG QEGVDSM AEE GTSDSN TGSE SNSAT VEEPP TDPI PEDEKK E, corresponding to C terminal amino acids 360-411 of Human BAF57/SMARCE1 (NP_003070.3)

  • 阳性对照
    • Human ovarian tumor. Mouse squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer recommended.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • 序列相似性Contains 1 HMG box DNA-binding domain.
    • 结构域The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAF57 antibody
      • BRG1 associated factor 57 antibody
      • BRG1-associated factor 57 antibody
      • Chromatin remodeling complex BRG1 associated factor 57 antibody
      • FLJ35648 antibody
      • SMARCE 1 antibody
      • SMARCE1 antibody
      • SMCE1_HUMAN antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
      see all

    Anti-BAF57/SMARCE1 antibody 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human ovarian tumor using 1/250 ab84420, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84420, followed by DAB staining.

    Anti-BAF57/SMARCE1 antibody (ab84420)参考文献

    ab84420 has not yet been referenced specifically in any publications.

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