Anti-Bad (phospho S112)抗体(ab1435)


  • 产品名称Anti-Bad (phospho S112)抗体
    参阅全部 Bad 一抗
  • 描述
    兔多克隆抗体to Bad (phospho S112)
  • 特异性The antibody detects Bad only when phosphorylated at Ser 112.
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human



Our Abpromise guarantee covers the use of ab1435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.


  • 功能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 组织特异性Expressed in a wide variety of tissues.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻译后修饰Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • 细胞定位Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

Anti-Bad (phospho S112) antibody 图像

  • All lanes : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml

    Lane 2 : COS cell lysate. Anti-BAD antibody used as a loading control.
    Lane 3 : COS cell lysate. Anti-BAD antibody used as a loading control.
    Lane 4 : COS cell lysate. Anti-BAD antibody used as a loading control.
    Lane 5 : COS cell lysate. Anti-BAD antibody used as a loading control.

    Performed under reducing conditions.

    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-Bad (phospho S112) antibody (ab1435)参考文献

ab1435 has not yet been referenced specifically in any publications.

Product Wall

The supplier of this antibody has informed me that ab1435 should be used at a concentration of 10 ug/ml for IP. I apologize for the error on the datasheet and have corrected it. I recommend trying ab1435 at 10 ug/ml and please let me know how this work...

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