概述

  • 产品名称
  • 描述
    兔多克隆抗体to Bad
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cat, Dog, Chimpanzee, Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human Bad aa 1-50. The exact sequence is proprietary. (NP_004313.1).
    Database link: Q92934

  • 阳性对照
    • IHC-P: Human ovarian carcinoma and prostate carcinoma tissues; Mouse renal cell carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab226461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.

Or 1/50 - 1/500 if using a fluorescent secondary antibody.

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

靶标

  • 功能
    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 组织特异性
    Expressed in a wide variety of tissues.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻译后修饰
    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • 细胞定位
    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

图片

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Bad using ab226461 at 1/500 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Bad using ab226461 at 1/100 dilution in immunohistochemical analysis.

    Detection: Red-fluorescent Goat Anti-Rabbit IgG (highly cross-absorbed) at 1/100 dilution.

  • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for Bad using ab226461 at 1/100 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Bad using ab226461 at 1/500 dilution in immunohistochemical analysis.

    Detection: DAB staining.

文献

ab226461 has not yet been referenced specifically in any publications.

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