概述

  • 产品名称Anti-Bad抗体
    参阅全部 Bad 一抗
  • 描述
    兔多克隆抗体to Bad
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human Bad (NP_004313.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab114105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
    • 组织特异性Expressed in a wide variety of tissues.
    • 序列相似性Belongs to the Bcl-2 family.
    • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 翻译后修饰Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
    • 细胞定位Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    Anti-Bad antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse renal cell carcinoma (right) tissue labelling Bad with ab114105 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human Bad by Western blot of Immunoprecipitate.
      Bad was immunoprecipitated from HeLa whole cell lysate, using ab114105 at 10 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114105 was used for subsequent blotting at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-Bad antibody (ab114105)参考文献

    ab114105 has not yet been referenced specifically in any publications.

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