Anti-Bad抗体[64103] (ab10520)

概述

  • 产品名称
    Anti-Bad抗体[64103]
    参阅全部 Bad 一抗
  • 描述
    大鼠单克隆抗体[64103] to Bad
  • 特异性
    This antibody will also detect a 32 kDa band that comigrates with recombinant Bad on immunoblots.
  • 经测试应用
    适用于: Dot blotmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Recombinant N-Termanal fragment (Mouse).

性能

应用

Our Abpromise guarantee covers the use of ab10520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
  • 应用说明
    Dot: a working antibody concentration of approximately 1 µg/ml is recommended using extracts from 2 x 106 mouse cytotoxic T cell line (CTLL2) or L929 fibroblast cells in an enhanced chemiluminescent (ECL) assay.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
    • 组织特异性
      Expressed in a wide variety of tissues.
    • 序列相似性
      Belongs to the Bcl-2 family.
    • 结构域
      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 翻译后修饰
      Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
    • 细胞定位
      Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    Anti-Bad antibody [64103] (ab10520)参考文献

    ab10520 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab10520.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册