Anti-bacterial Alkaline Phosphatase抗体(Biotin) (ab7321)

概述

  • 产品名称
    Anti-bacterial Alkaline Phosphatase抗体(Biotin)
  • 描述
    兔多克隆抗体to bacterial Alkaline Phosphatase (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
  • 种属反应性
    No reactivity occurs against mammalian forms of Alkaline Phosphatase.
  • 免疫原

    Full length protein (E. coli).

  • 常规说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
Immunomicroscopy
WB
Dot blot
ELISA
  • 应用说明
    Immunoblotting (western or dot blot)
    ELISA (1:4,000 to 1:20,000)
    Immuno microscopy
    Immunohistochemistry
    as well as other peroxidase-antibody based enzymatic assays.
  • 靶标

    • 相关性
      Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
    • 细胞定位
      Periplasm
    • 别名
      • Alkaline phosphatase antibody
      • APase antibody
      • BAP antibody
      • PhoA antibody

    Anti-bacterial Alkaline Phosphatase antibody (Biotin) (ab7321)参考文献

    ab7321 has not yet been referenced specifically in any publications.

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