Anti-Bacillus anthracis spore antigen抗体[SA26] (ab2281)

概述

  • 产品名称
    Anti-Bacillus anthracis spore antigen抗体[SA26]
  • 描述
    小鼠单克隆抗体[SA26] to Bacillus anthracis spore antigen
  • 特异性
    No cross reactivity has been found in ELISA with B.anthracis vegetative cells, B.globiglii, B.subtilis and B.cereus spores.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 免疫原

    Bacillusanthracis spore extract.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This product has been shown to be 95% pure by 7.5 % SDS-PAGE.
  • 克隆
    单克隆
  • 克隆编号
    SA26
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG2a
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Can be used in a sandwich ELISA along with ab1994.

WB Use a concentration of 2 µg/ml. Detects a band of approximately 92-94 kDa.

(Under non-reducing conditions)

靶标

  • 相关性
    Bacillus anthracis is an aerobic spore forming bacterium that causes anthrax, an acute infectious disease of cattle, sheep, goats, camels, antelopes and other herbivores. Infection can occur in humans when they are exposed to infected animals or tissue from infected animals via three routes: cutaneous, inhalation and gastrointestinal. B. anthracis spores persist in the soil for many years as they are resistant to heat, cold, radiation, desiccation, and disinfectants. In vivo, B anthracis produces a polypeptide (polyglutamic acid) capsule that protects it from phagocytosis. The virulence factors of B anthracis are its capsule and three component toxin, both encoded on plasmids.

图片

  • Lane 1 : Anti-Bacillus anthracis spore antigen antibody [SA26] (ab2281)
    Lane 2 : Clone SA27

    Lane 1 : B. anthracis spore
    Lane 2 : B. anthracis spore


    Performed under reducing conditions.

    10% SDS-PAGE

实验方案

文献

This product has been referenced in:
  • Sanz P  et al. Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging. Infect Immun 76:1036-47 (2008). Read more (PubMed: 18195028) »
  • Williams DD & Turnbough CL Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J Bacteriol 186:566-9 (2004). Read more (PubMed: 14702325) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your patience.

I have hard back from the supplying lab's specialistwith more information regarding the specificity of the ab2281 [SA26]:

Here are the comments from our specialist:

In different formats SA26 w...

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Thank you for contacting us.

I hear back from the supplying lab:

According to our info ab2281 [SA26] is against anthrax spore so at the moment I unfortunately do not have any other info to give. As soon as I got the details for the...

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Thank you for your patience.

The lab we receive this product from, let me know the following today:

"I discussed with our specialist and he confirmed that according to our info ab2281[SA26] is against anthrax spore".

The ...

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Thank you for your enquiry. The concentration of the lot is 6.4 mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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