Anti-Bacillus anthracis spore antigen抗体[SA26] (ab2281)

概述

  • 产品名称Anti-Bacillus anthracis spore antigen抗体[SA26]
  • 描述
    小鼠单克隆抗体[SA26] to Bacillus anthracis spore antigen
  • 特异性No cross reactivity has been found in ELISA with B.anthracis vegetative cells, B.globiglii, B.subtilis and B.cereus spores.
  • 经测试应用适用于: ELISA, WBmore details
  • 免疫原

    Bacillusanthracis spore extract.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This product has been shown to be 95% pure by 7.5 % SDS-PAGE.
  • 克隆单克隆
  • 克隆编号SA26
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution. Can be used in a sandwich ELISA along with ab1994.
    WB: Use at a concentration of 2 µg/ml. (Under non-reducing conditions) Detects a band of approximately 92-94 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Bacillus anthracis is an aerobic spore forming bacterium that causes anthrax, an acute infectious disease of cattle, sheep, goats, camels, antelopes and other herbivores. Infection can occur in humans when they are exposed to infected animals or tissue from infected animals via three routes: cutaneous, inhalation and gastrointestinal. B. anthracis spores persist in the soil for many years as they are resistant to heat, cold, radiation, desiccation, and disinfectants. In vivo, B anthracis produces a polypeptide (polyglutamic acid) capsule that protects it from phagocytosis. The virulence factors of B anthracis are its capsule and three component toxin, both encoded on plasmids.

    Anti-Bacillus anthracis spore antigen antibody [SA26] 图像

    • ab2281 at 2mg/ml staining Bacillus anthracis spore antigen.

      Lane 1 - clone SA26
      Lane 2 - clone SA27
      Lane 3 - monoclonal antibody against protective antigen of Bacillus Anthracis -C3 (ab8240)
      Lane 4 - monoclonal antibody against protective antigen of Bacillus Anthracis -BAP0104 (ab1991)
      Lane 5 - control a-IgG(mouse) - HRP
      Lane 6 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244).
      Lane 7 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244)
      Lane 8 - control a-IgG (rabbit) - HRP
      Lane 9 - B.anthracis spores
      Lane 10 - Molecular mass standards from top to bottom 94K, 67K, 43K, 30K, 20K; Amidoblack stain.

      After washing, the nitrocellulose was incubated with anti-mouse IgG - HRP or anti-rabbit IgG-HRP conjugates at working dilutions 1:25000 and 1:500 respectively. Later on the substrate solution, containing diaminobenzidine with 4-chloro-1-naphtol was added.

    Anti-Bacillus anthracis spore antigen antibody [SA26] (ab2281)参考文献

    This product has been referenced in:
    • Sanz P  et al. Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging. Infect Immun 76:1036-47 (2008). Read more (PubMed: 18195028) »
    • Williams DD & Turnbough CL Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J Bacteriol 186:566-9 (2004). Read more (PubMed: 14702325) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your patience.

    I have hard back from the supplying lab's specialistwith more information regarding the specificity of the ab2281 [SA26]:

    Here are the comments from our specialist:

    In different formats SA26 w...

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    Thank you for contacting us.

    I hear back from the supplying lab:

    According to our info ab2281 [SA26] is against anthrax spore so at the moment I unfortunately do not have any other info to give. As soon as I got the details for the...

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    Thank you for your patience.

    The lab we receive this product from, let me know the following today:

    "I discussed with our specialist and he confirmed that according to our info ab2281[SA26] is against anthrax spore".

    The ...

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    Thank you for your enquiry. The concentration of the lot is 6.4 mg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"