Anti-Bacillus anthracis lethal factor抗体[BAL0105] (ab8242)

概述

  • 产品名称Anti-Bacillus anthracis lethal factor抗体[BAL0105]
    参阅全部 Bacillus anthracis lethal factor 一抗
  • 描述
    小鼠单克隆抗体[BAL0105] to Bacillus anthracis lethal factor
  • 特异性Lethal factor antigen of Bacillus anthracis.
  • 经测试应用适用于: ELISAmore details
  • 免疫原

    Highly purified lethal factor antigen of Bacillus anthracis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS with 0.1% sodium azide
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号BAL0105
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
    • 细胞定位secreted
    • 别名
      • Anthrax lethal toxin endopeptidase component antibody
      • Anthrax LF antibody
      • Lef antibody
      • Lethal factor antibody
      • LF antibody
      see all

    实验方案

    Anti-Bacillus anthracis lethal factor antibody [BAL0105] (ab8242)参考文献

    ab8242 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    I have had the oportunity to look over the data regarding the antibodies we had talked about this morning. I am confident that your assessment of those products we correct as ab8242 and ab24451 have been created ...

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    Thank you for your enquiry. Our source has replied: "The normal presentation of the antibodies is non-conjugated, but we are also offering them in conjugated form. We are normally not using anti-mouse IgG secondary antibodies, we are using hrp-conjug...

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    I double checked with the supplier of these antibodies, and the calibration curve located on the datasheet for ab8242 is incorrect. The curve is not for clones BAL0105-BAL0106 (abs8242-8243). I apologize for this error and the datasheet will be correct...

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    Unfortunately, we have no information whether this antibody (ab8242) is compatible with the Sterne strain.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"