Anti-Bacillus anthracis lethal factor抗体(ab13807)

概述

  • 产品名称Anti-Bacillus anthracis lethal factor抗体
    参阅全部 Bacillus anthracis lethal factor 一抗
  • 描述
    兔多克隆抗体to Bacillus anthracis lethal factor
  • 特异性The antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    Anthrax LF (Lethal Factor) produced by Bacillus anthracis
  • 免疫原

    Synthetic peptide corresponding to amino acids 779-792 of Anthrax LF

  • 阳性对照
    • 0.1 ug recombinant protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.2% gelatin, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 90 kDa.

靶标

  • 相关性The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • 细胞定位secreted
  • 别名
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

Anti-Bacillus anthracis lethal factor antibody 图像

  • Western blot analysis of Anthrax LF in recombinant protein using ab13807 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Anti-Bacillus anthracis lethal factor antibody (ab13807)参考文献

ab13807 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Both of these antibodies are sold as sera and therefore concentration of specific antibody has not been determined. Recommended dilutions are given on the online datasheets, and I would recommend referring to those. If you h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"