Anti-Bacillus anthracis lethal factor抗体[5F502.2] (ab13814)

概述

  • 产品名称Anti-Bacillus anthracis lethal factor抗体[5F502.2]
    参阅全部 Bacillus anthracis lethal factor 一抗
  • 描述
    小鼠单克隆抗体[5F502.2] to Bacillus anthracis lethal factor
  • 特异性The antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    Anthrax LF (Lethal Factor) produced by Bacillus anthracis
  • 免疫原

    Synthetic peptide, corresponding to amino acids 779-792 of Anthrax LF

  • 阳性对照
    • 0.1 ug recombinant protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号5F502.2
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 90 kDa.

靶标

  • 相关性The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • 细胞定位secreted
  • 别名
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

Anti-Bacillus anthracis lethal factor antibody [5F502.2] 图像

  • Western blot analysis of Anthrax LF in recombinant protein using ab13814 at 0.1 mg/ml.

Anti-Bacillus anthracis lethal factor antibody [5F502.2] (ab13814)参考文献

This product has been referenced in:
  • Geddie ML  et al. Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. J Biol Chem 280:35641-6 (2005). Read more (PubMed: 16118206) »

See 1 Publication for this product

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Thank you for your enquiry. To test the antibodies ab13814, ab13805, ab13806 and ab13807 0.1 ug recombinant protein was loaded per lane. Please let me know if you require further information,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"