Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
Synthetic peptide corresponding to a region within internal sequence amino acids 792-841 (SGRRLEGTDP GTFSERGPPL EPRSQTVTVD FCQEMTDKCT TDEQPRKDYT) of Human BACH2 (NP_068585).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2.5 µg/ml.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.Can be blocked with Human BACH2 peptide (ab111653). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.
Belongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain.
Information by UniProt
- Bach 2 antibody
- Bach2 antibody
- BACH2_HUMAN antibody
Western blot - BACH2 antibody (ab83364)
Anti-BACH2 antibody (ab83364) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
93 kDaObserved band size :
>90 kDa (why is the actual band size different from the predicted?
)Additional bands at :
60 kDa,80 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-BACH2 antibody(ab83364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling BACH2 with ab83364 at 2.5 µg/ml.
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