概述

  • 产品名称
  • 描述
    兔多克隆抗体to BACH2
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 792-841 (SGRRLEGTDP GTFSERGPPL EPRSQTVTVD FCQEMTDKCT TDEQPRKDYT) of Human BACH2 (NP_068585).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.Can be blocked with Human BACH2 peptide (ab111653). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

  • 功能
    Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.
  • 组织特异性
    B-cell specific.
  • 序列相似性
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bach 2 antibody
    • Bach2 antibody
    • BACH2_HUMAN antibody
    • Basic leucine zipper transcription factor 2 antibody
    • BTB and CNC homolog 2 antibody
    • BTB and CNC homology 1 basic leucine zipper transcription factor 2 antibody
    • BTB domain and CNC homolog 2 antibody
    • BTBD25 antibody
    • OTTHUMP00000016867 antibody
    • OTTHUMP00000016868 antibody
    • OTTHUMP00000040591 antibody
    • Transcription regulator protein BACH2 antibody
    see all

图片

  • Anti-BACH2 antibody (ab83364) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling BACH2 with ab83364 at 2.5 µg/ml.

文献

This product has been referenced in:
  • Hunter JE  et al. The NF-?B subunit c-Rel regulates Bach2 tumour suppressor expression in B-cell lymphoma. Oncogene 35:3476-84 (2016). Read more (PubMed: 26522720) »
  • Duan Z  et al. Attenuation of antigenic immunogenicity by kynurenine, a novel suppressive adjuvant. Hum Vaccin Immunother 10:1295-305 (2014). Read more (PubMed: 24583631) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (B Cell)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
50 µg
Specification
B Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dipmoy Nath

Verified customer

提交于 Mar 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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