Anti-BACH1/BRIP1抗体- N-terminal (ab151509)
Key features and details
- Rabbit polyclonal to BACH1/BRIP1 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BACH1/BRIP1抗体- N-terminal
参阅全部 BACH1/BRIP1 一抗 -
描述
兔多克隆抗体to BACH1/BRIP1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within N terminal amino acids 52-291 of Human BACH1/BRIP1.
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阳性对照
- HeLa whole cell lysate (ab150035); 293T cells; Human SAS xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 140 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 140 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. -
组织特异性
Ubiquitously expressed, with highest levels in testis. -
疾病相关
Defects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
序列相似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain. -
结构域
4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400). -
翻译后修饰
Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 83990 Human
- Omim: 605882 Human
- SwissProt: Q9BX63 Human
- Unigene: 128903 Human
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别名
- ATP dependent RNA helicase BRIP1 antibody
- ATP-dependent RNA helicase BRIP1 antibody
- BACH 1 antibody
see all
图片
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Anti-BACH1/BRIP1 antibody - N-terminal (ab151509) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 140 kDa
5% SDS-PAGE. -
Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling BACH1/BRIP1 with ab151509 at 1/500 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling BACH1/BRIP1 with ab151509 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33343.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab151509 被引用在 3 文献中.
- Gupta I et al. BRIP1 overexpression is correlated with clinical features and survival outcome of luminal breast cancer subtypes. Endocr Connect 7:65-77 (2018). PubMed: 29138235
- Barthelemy J et al. FANCJ is essential to maintain microsatellite structure genome-wide during replication stress. Nucleic Acids Res 44:6803-16 (2016). WB ; Human . PubMed: 27179029
- Balacescu O et al. Gene expression profiling reveals activation of the FA/BRCA pathway in advanced squamous cervical cancer with intrinsic resistance and therapy failure. BMC Cancer 14:246 (2014). IHC . PubMed: 24708616