概述

  • 产品名称
    Anti-BACH1/BRIP1抗体
    参阅全部 BACH1/BRIP1 一抗
  • 描述
    兔多克隆抗体to BACH1/BRIP1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    SKDFTNNDMNQGT

    , corresponding to amino acids 92-104 of Human BACH 1.

  • 常规说明
    Expiration date is one (1) year from date of opening.

性能

应用

Our Abpromise guarantee covers the use of ab3502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Detects a band of approximately 105 kDa (predicted molecular weight: 140 kDa).

靶标

  • 功能
    DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
  • 组织特异性
    Ubiquitously expressed, with highest levels in testis.
  • 疾病相关
    Defects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 序列相似性
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
  • 结构域
    4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
  • 翻译后修饰
    Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase BRIP1 antibody
    • ATP-dependent RNA helicase BRIP1 antibody
    • BACH 1 antibody
    • BRAC 1 Associated C Terminal Helicase 1 antibody
    • BRCA 1 Interacting Protein 1 antibody
    • BRCA1 binding helicase like protein BACH1 antibody
    • BRCA1 interacting protein C terminal helicase 1 antibody
    • BRCA1-associated C-terminal helicase 1 antibody
    • BRCA1-interacting protein 1 antibody
    • BRCA1-interacting protein C-terminal helicase 1 antibody
    • BRCA1/BRCA2 associated helicase 1 antibody
    • BRIP 1 antibody
    • BRIP1 antibody
    • FANCJ antibody
    • FANCJ_HUMAN antibody
    • Fanconi anemia group J protein antibody
    • FLJ90232 antibody
    • MGC126521 antibody
    • MGC126523 antibody
    • OF antibody
    • Protein FACJ antibody
    see all

Anti-BACH1/BRIP1 antibody 图像

  • All lanes : Anti-BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution

    Lane 1 : 293 whole cell lysate
    Lane 2 : 293 whole cell lysate with peptide immunogen

    Lysates/proteins at 35 µg per lane.

    Secondary
    IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-BACH1/BRIP1 antibody (ab3502)参考文献

ab3502 has not yet been referenced specifically in any publications.

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