概述

  • 产品名称Anti-BACE1抗体[EPR3956]
    参阅全部 BACE1 一抗
  • 描述
    兔单克隆抗体[EPR3956] to BACE1
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BACE1.

  • 阳性对照
    • WB: SH-SY5Y cell lysate and human fetal, mouse and rat brain tissue lysates. IP: Human fetal brain tissue lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab108394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
    • 组织特异性Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
    • 序列相似性Belongs to the peptidase A1 family.
    • 结构域The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
    • 翻译后修饰Glycosylated.
    • 细胞定位Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APP beta secretase antibody
      • Asp 2 antibody
      • ASP2 antibody
      • Aspartyl protease 2 antibody
      • BACE 1 antibody
      • BACE antibody
      • BACE1 antibody
      • BACE1_HUMAN antibody
      • Beta secretase 1 antibody
      • Beta secretase antibody
      • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
      • Beta site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta site amyloid precursor protein cleaving enzyme antibody
      • Beta site APP cleaving enzyme 1 antibody
      • Beta site APP cleaving enzyme antibody
      • Beta-secretase 1 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta-site APP cleaving enzyme 1 antibody
      • FLJ90568 antibody
      • HSPC104 antibody
      • Memapsin 2 antibody
      • Memapsin-2 antibody
      • Memapsin2 antibody
      • Membrane associated aspartic protease 2 antibody
      • Membrane-associated aspartic protease 2 antibody
      • Transmembrane aspartic proteinase Asp2 antibody
      see all

    Anti-BACE1 antibody [EPR3956] 图像

    • All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 56 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 56 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

      Lane 1 (input): human fetal brain whole tissue lysate (10µg)

      Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10µg).

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108394 in human fetal brain whole tissue lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 56 kDa

    Anti-BACE1 antibody [EPR3956] (ab108394)参考文献

    This product has been referenced in:
    • Yang C  et al. Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer transgenic mouse model. Neuropeptides 48:233-8 (2014). WB ; Mouse . Read more (PubMed: 24813062) »
    • Hitt B  et al. ß-Site Amyloid Precursor Protein (APP)-cleaving Enzyme 1 (BACE1)-deficient Mice Exhibit a Close Homolog of L1 (CHL1) Loss-of-function Phenotype Involving Axon Guidance Defects. J Biol Chem 287:38408-25 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22988240) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your reply.

    I am sending you a new vial of ab2077, your new order number is ** and you should have the new antibody by 10.30am tomorrow morning. If there is anything else I can help you with, or if you have any issues with the n...

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    Thank you for your reply.

    If sending you a new aliquot of the cell lysate would help you to complete your experiments, then I would be happy to do so. However, I am confused as to why you think that the current vial of ab2077 that you have is...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"