概述

  • 产品名称Anti-BACE1抗体
    参阅全部 BACE1 一抗
  • 描述
    兔多克隆抗体to BACE1
  • 特异性Multiple isoforms of this protein are known to exist; this antibody will detect all isoforms. The glycosylated isoform may also be observed at 70kDa in SDS-PAGE.
  • 经测试应用适用于: ICC, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).

  • 阳性对照
    • Human brain tissue lysate, 3T3 lysate.
  • 常规说明

    Beta-site APP Cleaving Enzyme.

性能

应用

Our Abpromise guarantee covers the use of ab2077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with BACE1 peptide (ab7883). We recommend overnight incubation at 4C and using 5% skim milk to block.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • 组织特异性Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • 序列相似性Belongs to the peptidase A1 family.
  • 结构域The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • 翻译后修饰Glycosylated.
  • 细胞定位Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Anti-BACE1 antibody 图像

  • Immunohistochemistry of BACE in mouse brain tissue with BACE antibody at 2.5 µg/ml.

  • All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml

    Lane 1 : Human brain tissue lysate with absence of blocking peptide
    Lane 2 : Human brain tissue lysate with BACE1 peptide (ab7883)
    Lane 3 : Mouse 3T3 cell lysate


    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

Anti-BACE1 antibody (ab2077)参考文献

This product has been referenced in:
  • Yan X  et al. Ginsenoside Rd. promotes non-amyloidogenic pathway of amyloid precursor protein processing by regulating phosphorylation of estrogen receptor alpha. Life Sci N/A:N/A (2016). Read more (PubMed: 27825720) »
  • Xu Z  et al. Age-dependent postoperative cognitive impairment and Alzheimer-related neuropathology in mice. Sci Rep 4:3766 (2014). WB ; Mouse . Read more (PubMed: 24441878) »

See all 20 Publications for this product

Product Wall

Thank you for contacting us.

Your credit note ID is xxxxx.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

Read More

Thank you for your message and for providing this further information.

I am sorry to confirm that we can not make any further suggestions in this case to improve the results. It seems that you regrettably received a bad vial. For that reason I...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-BACE1 antibody (ab2077) .

The details you have kindly provided will enable us ...

Read More

Thank you for your reply.

I am sending you a new vial of ab2077, your new order number is ** and you should have the new antibody by 10.30am tomorrow morning. If there is anything else I can help you with, or if you have any issues with the n...

Read More

Thank you for your reply.

If sending you a new aliquot of the cell lysate would help you to complete your experiments, then I would be happy to do so. However, I am confused as to why you think that the current vial of ab2077 that you have is...

Read More

Thank you for your reply.

I am sorry about the issues you have been having with ab2077 in western blot. I cannot send you all of the reagents that you are requesting, I can either send 1 free antibody as a replacement for ab2077, or I can pro...

Read More

Thank you for calling Abcam yesterday.

I am sorry about the issues you have been having with ab2077 in western blot. Having talked to my colleagues, I think the best way to move forward with this is to either replace the antibody that you cur...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (murine glioma cell line C6)
Specification murine glioma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2011

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"