Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: BSA, Glycerol, Sodium chloride, HEPES, Potassium chloride, chaotropic agents, pH 7.4
The buffer contains primary amines and is not suitable for conjugation via NH2 group.
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Immunogen affinity purified
Purified against immobilized antigen based affinity chromatography which yielded an epitope specific antibody.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent dilution.
WB: >1/500. Detects a band of approximately 73 kDa
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In higher vertebrates the patched (Ptc) also acts as a tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc removes the negative interaction of Ptc, and allows the signaling by smoothened. It has been shown that during head morphogenesis in D. melanogaster, Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon, and activation of cell proliferation in the eye antennal disc. The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects.