概述
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产品名称Anti-BAALC抗体
参阅全部 BAALC 一抗 -
描述山羊多克隆抗体to BAALC
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宿主Goat
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特异性ab77101 is expected to recognise BAALC isoform 1 (NP_079088.1) only.
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Mouse
预测可用于: Rat, Human, Pig
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免疫原
Synthetic peptide: C-KQNGLQTTEA KRDAK, corresponding to internal sequence amino acids 101-115 of Human BAALC (NP_079088.1)
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阳性对照
- Human and mouse brain lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab77101 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab77101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.
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组织特异性Expressed by neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. Isoform 2 and isoform 6 are expressed in the brain. Isoform 2 shows a low expression in neuroectoderm-derived tissues such as adrenal gland and no expression in bone marrow, peripheral blood lymphocytes or lymph nodes, or in tumors or cancer cell lines of nonneural tissue origin.
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翻译后修饰Palmitoylation and myristoylation target the protein to the lipid rafts.
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细胞定位Cytoplasm. Membrane. Postsynaptic lipid rafts.
- Information by UniProt
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数据库链接
- Entrez Gene: 79870 Human
- Entrez Gene: 118452 Mouse
- Entrez Gene: 100170128 Pig
- Entrez Gene: 140720 Rat
- Omim: 606602 Human
- SwissProt: Q8WXS3 Human
- SwissProt: Q8VHV1 Mouse
- SwissProt: Q8WNE9 Pig
see all -
别名
- BAALC antibody
- BAALC_HUMAN antibody
- Brain and acute leukemia cytoplasmic antibody
see all
图片
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Western blot - Anti-BAALC antibody (ab77101)Anti-BAALC antibody (ab77101) at 0.03 µg/ml + mouse brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
An additional band of unknown identity was also consistently observed at 48 kDa in both human and mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide.
文献
ab77101 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"