概述

  • 产品名称
  • 描述
    山羊多克隆抗体to BAALC
  • 特异性
    ab77101 is expected to recognise BAALC isoform 1 (NP_079088.1) only.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human, Pig
  • 免疫原

    Synthetic peptide: C-KQNGLQTTEA KRDAK, corresponding to internal sequence amino acids 101-115 of Human BAALC (NP_079088.1)

  • 阳性对照
    • Human and mouse brain lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab77101 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.
    • 组织特异性
      Expressed by neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. Isoform 2 and isoform 6 are expressed in the brain. Isoform 2 shows a low expression in neuroectoderm-derived tissues such as adrenal gland and no expression in bone marrow, peripheral blood lymphocytes or lymph nodes, or in tumors or cancer cell lines of nonneural tissue origin.
    • 翻译后修饰
      Palmitoylation and myristoylation target the protein to the lipid rafts.
    • 细胞定位
      Cytoplasm. Membrane. Postsynaptic lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAALC antibody
      • BAALC_HUMAN antibody
      • Brain and acute leukemia cytoplasmic antibody
      • Brain and acute leukemia cytoplasmic protein antibody
      • FLJ12015 antibody
      see all

    Anti-BAALC antibody 图像

    • Anti-BAALC antibody (ab77101) at 0.03 µg/ml + mouse brain lysate (in RIPA buffer) at 35 µg

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    Anti-BAALC antibody (ab77101)参考文献

    ab77101 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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