The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.
Expressed by neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. Isoform 2 and isoform 6 are expressed in the brain. Isoform 2 shows a low expression in neuroectoderm-derived tissues such as adrenal gland and no expression in bone marrow, peripheral blood lymphocytes or lymph nodes, or in tumors or cancer cell lines of nonneural tissue origin.
Palmitoylation and myristoylation target the protein to the lipid rafts.