Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 30-60 of Human B9D2.
Mouse spleen tissue lysate; Human lung carcinoma tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103961 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500. Predicted molecular weight: 19 kDa.
B9D2 is a B9 domain protein, which are exclusively found in ciliated organisms. The B9D2 gene is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects.
Cytoplasm; cytoskeleton; cilium basal body; cilium axoneme. Nucleus.
B9 domain containing protein 2 antibody
B9 protein domain 2 antibody
ICIS 1 antibody
Western blot - B9D2 antibody (ab103961)
Anti-B9D2 antibody (ab103961) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B9D2 antibody (ab103961)
ab103961, at 1/50 dilution, staining B9D2 in formalin-fixed, paraffin-embedded Human lung carcinoma by Immunohistochemistry, using a peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"