antigen sequence, corresponding to amino acids 4-67 (ASPSVFLLMV NGQVESAQFP EYDDLYCKYC FVYGQDWAPT AGLEEGISQI TSKSQDVRQA LVWN) of Human B9D1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the B9D family.
Contains 1 B9 domain.
Information by UniProt
- AW045994 antibody
- B9 antibody
- B9 domain containing protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-B9D1 antibody (ab121726)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121726 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B9D1 antibody (ab121726)
ab121726 at 1/250 dilution staining B9D1 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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