• 产品名称Anti-B7-H6抗体
  • 描述
    兔多克隆抗体to B7-H6
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 38-169 of Human B7-H6.

  • 阳性对照
    • Human gallbladder tissue.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab121794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Triggers NCR3-dependent natural killer cell activation.
  • 组织特异性Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
  • 序列相似性Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B7 homolog 6 antibody
    • B7-H6 antibody
    • B7H6 antibody
    • B7H6_HUMAN antibody
    • DKFZp686O24166 antibody
    • Natural cytotoxicity triggering receptor 3 ligand 1 antibody
    • NCR3LG1 antibody
    • Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167 antibody
    see all

Anti-B7-H6 antibody 图像

  • Anti-B7-H6 antibody (ab121794) + HL-60 cell lysate

    Predicted band size : 51 kDa
  • ab121794, at 1/50 dilution, staining B7-H6 in paraffin-embedded Human gallbladder tissue, by Immunohistochemistry.
  • ab121794 at 1/50 dilution staining a cell-clot mixture of JVM (B7-H6 neg) and Hela (B7-H6 pos) (5:1) cells by IHC-P. The cell matrix section was subjected to heat-mediated antigen retrieval using 10 mM Citrate Buffer pH6 before being blocked with 10% horse serum for 30 minutes at 23°C and then being incubated with ab121794 (1/50 dilution) for 16 hours at 4°C. The secondary used was a Biotin conjugated goat anti-rabbit polyclonal (1/200 dilution).

    See Abreview

Anti-B7-H6 antibody (ab121794)参考文献

ab121794 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (JVM:HeLa (5:1) cell clot)
Specification JVM:HeLa (5:1) cell clot
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer pH6
Permeabilization No
Blocking step Horse serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Nov 30 2012

Thank you for contacting us.

I have discussion with our IT department - unfortunately they do not save the previous versions of datasheets when updating them. The online datasheets along with pdf's gets updated every 15-20 days which is necess...

Read More

Thank you for your message which has been forwarded to me as my colleague Claudia is not in the office.

I apologise for the delay in resonding. We are still waiting for further information regarding your offer of testing from our business de...

Read More

Thank you for contacting us.

Whenthe laboratory characterized thisantibody, there was almost no literature available to compare the staining results with, but now there is some new literature describing the localization of the target protein....

Read More

Vielen Dank für Ihre Anfrage.

Bisher wurde der ab121794 Anti-B7-H6 antibody leider nicht in ELISA getestet, und auch eine mögliche Kreuzreaktionen mit anderen B7-H Molekülen wurde nicht experimentell untersucht.

Der Antikörper wur...

Read More