ab74825 detects endogenous levels of total B4GALT3 protein.
Synthesized peptide derived from an internal region of human B4GALT3
WB: extracts from HepG2 cells, HeLa cells and Jurkat cells
IHC-P: human liver carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
Use at an assay dependent concentration.
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.
Protein modification; protein glycosylation.
Belongs to the glycosyltransferase 7 family.
Golgi apparatus > Golgi stack membrane. Trans cisternae of Golgi stack.
Information by UniProt
4-galactosyltransferase 3 antibody
4-GalTase 3 antibody
Western blot - B4GALT3 antibody (ab74825)
All lanes : Anti-B4GALT3 antibody (ab74825) at 1/500 dilution Lane 1 : extracts from HepG2 cells Lane 2 : extracts from HeLa cells Lane 3 : extracts from Jurkat cells Lane 4 : extracts from Jurkat cells with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 44 kDa Observed band size : 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B4GALT3 antibody (ab74825)
ab74825, at a dilution of 1/50, staining B4GALT3 in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
This product has been referenced in:
et al. B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1. Cell Death Dis 4:e654 (2013).
Read more (PubMed: 23744354) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"