Anti-B4GALT1抗体(ab121326)

概述

  • 产品名称Anti-B4GALT1抗体
    参阅全部 B4GALT1 一抗
  • 描述
    兔多克隆抗体to B4GALT1
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 50-163 of Human B4GALT1 (P15291).

  • 阳性对照
    • Human prostate tissue; U-251 MG cell lysate; A-431 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
    The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
  • 组织特异性Ubiquitously expressed, but at very low levels in fetal and adult brain.
  • 通路Protein modification; protein glycosylation.
  • 疾病相关Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • 序列相似性Belongs to the glycosyltransferase 7 family.
  • 翻译后修饰The soluble form derives from the membrane forms by proteolytic processing.
  • 细胞定位Golgi apparatus > Golgi stack membrane. Cell membrane. Cell surface. Found in trans cisternae of Golgi; Golgi apparatus > Golgi stack membrane. Found in trans cisternae of Golgi and Secreted. Soluble form found in body fluids.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody
    • 4-galactosyltransferase 1 antibody
    • 4-GalTase 1 antibody
    • b4Gal-T1 antibody
    • B4galt1 antibody
    • B4GT1_HUMAN antibody
    • Beta-1 antibody
    • Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase antibody
    • Beta4Gal-T1 antibody
    • CDG2D antibody
    • GGTB2 antibody
    • GT1 antibody
    • GTB antibody
    • Lactose synthase A protein antibody
    • N-acetyllactosamine synthase antibody
    • Nal synthase antibody
    • Processed beta-1 antibody
    • UDP-Gal:beta-GlcNAc beta-1 antibody
    • UDP-galactose:beta-N-acetylglucosamine beta-1 antibody
    see all

Anti-B4GALT1 antibody 图像

  • All lanes : Anti-B4GALT1 antibody (ab121326) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121326, at 1/125, staining B4GALT1 in paraffin embedded Human prostate tissue by Immunohistochemistry.
  • ab121326, at 4 µg/ml, staining B4GALT1 (green) in A-431 cells by Immunofluorescence.

Anti-B4GALT1 antibody (ab121326)参考文献

ab121326 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (A549)
Specification A549
Permeabilization No
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"