概述

  • 产品名称Anti-B3GALTL抗体
    参阅全部 B3GALTL 一抗
  • 描述
    兔多克隆抗体to B3GALTL
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from the C-terminus of human B3GALTL

  • 阳性对照
    • Human liver carcinoma tissue. Extracts from HepG2, Jurkat, MCF-7 or HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性B3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1; MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2; MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008).
  • 细胞定位Endoplasmic reticulum membrane; Single pass type II membrane protein.
  • 数据库链接
  • 别名
    • B3GlcT antibody
    • B3GTL antibody
    • Beta 1,3 galactosyltransferase like antibody
    • Beta 3 galactosyltransferase like antibody
    • Beta-1,3-glucosyltransferase antibody
    • Beta-3-glycosyltransferase-like antibody
    • Beta3Glc-T antibody
    • Beta3GlcT antibody
    • Galactosyltransferase antibody
    • GalT antibody
    see all

Anti-B3GALTL antibody 图像

  • All lanes : Anti-B3GALTL antibody (ab74824) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from MCF-7 cells
    Lane 4 : extracts from HUVEC cells
    Lane 5 : extracts from HUVEC cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
  • ab74824, at a dilution of 1/50, staining B3GALTL in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

Anti-B3GALTL antibody (ab74824)参考文献

ab74824 has not yet been referenced specifically in any publications.

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