Anti-B Raf (phospho T401)抗体[EPR2208Y] (ab68215)


  • 产品名称Anti-B Raf (phospho T401)抗体[EPR2208Y]
    参阅全部 B Raf 一抗
  • 描述
    兔单克隆抗体[EPR2208Y] to B Raf (phospho T401)
  • 特异性This antibody detects B Raf phosphorylated on threonine 401.
  • 经测试应用适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphospecific peptide corresponding to residues surrounding threonine 401 of human B Raf.

  • 阳性对照
    • PC-12 cell lysates (cells untreated or treated with TPA)
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.




Our Abpromise guarantee covers the use of ab68215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 85 kDa.
IP 1/20.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
    • 组织特异性Brain and testis.
    • 疾病相关Note=Defects in BRAF are found in a wide range of cancers.
      Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
      Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
      Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
      Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
      Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
      Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 RBD (Ras-binding) domain.
    • 细胞定位Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ95109 antibody
      • 94 kDa B raf protein antibody
      • B raf 1 antibody
      • B Raf proto oncogene serine threonine protein kinase antibody
      • B Raf proto oncogene, serine/threonine kinase antibody
      • B RAF1 antibody
      • B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
      • BRAF 1 antibody
      • BRAF antibody
      • BRAF_HUMAN antibody
      • BRAF1 antibody
      • cRmil antibody
      • MGC126806 antibody
      • MGC138284 antibody
      • Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
      • Murine sarcoma viral v raf oncogene homolog B1 antibody
      • NS7 antibody
      • Oncogen BRAF antibody
      • oncogene BRAF1 antibody
      • p94 antibody
      • Proto-oncogene B-Raf antibody
      • Proto-oncogene c-Rmil antibody
      • RAFB 1 antibody
      • RAFB1 antibody
      • RMIL antibody
      • Serine/threonine-protein kinase B-raf antibody
      • v raf murine sarcoma viral oncogene homolog B antibody
      • v raf murine sarcoma viral oncogene homolog B1 antibody
      • v-Raf murine sarcoma viral oncogene homolog B1 antibody
      see all

    Anti-B Raf (phospho T401) antibody [EPR2208Y] 图像

    • All lanes : Anti-B Raf (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution

      Lane 1 : PC-12 cell lysates (cells were untreated)
      Lane 2 : PC-12 cell lysates (cells were treated with TPA)

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa

    Anti-B Raf (phospho T401) antibody [EPR2208Y] (ab68215)参考文献

    ab68215 has not yet been referenced specifically in any publications.

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