Anti-B Cell Subset抗体[BB6-10A10] (Phycoerythrin) (ab25356)

概述

  • 产品名称Anti-B Cell Subset抗体[BB6-10A10] (Phycoerythrin)
    参阅全部 B Cell Subset 一抗
  • 描述
    小鼠单克隆抗体[BB6-10A10] to B Cell Subset (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Pig
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明


    ab25356 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by ab25356 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of s Ig low B cells in lymph nodes.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • 纯度Purified IgM
  • Primary antibody说明ab25356 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by ab25356 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of s Ig low B cells in lymph nodes.
  • 克隆单克隆
  • 克隆编号BB6-10A10
  • 同种型IgM
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2µg for 106 cells. ab154448-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性B-cells are lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.

Anti-B Cell Subset antibody [BB6-10A10] (Phycoerythrin) (ab25356)参考文献

ab25356 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

If you could send the data in which ever file format that you generally use, that would be great.

I do appreciate your patience in this matter and I hope we can get it resolved soon.

Thank you for your reply and for providing the protocol information.

Just to confirm that you re using “anti mouse serum Fc block”. Is this truly an Anti-Mouse? Also, are you adding the whole 50uL of diluted antibody to the cells?...

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Thank you for contacting Abcam earlier today.

The antibody ab25356 was tested on pig blood in flow cytometry. To date, we have never used frozen specimens in flow cytometry.Would you be ablesend a detailed protocol so thatI can send that to t...

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