概述

  • 产品名称Anti-AZI2抗体
    参阅全部 AZI2 一抗
  • 描述
    兔多克隆抗体to AZI2
  • 特异性ab65242 detects endogenous levels of total AZI2 protein.
  • 经测试应用适用于: ELISA, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from N terminal of human AZI2

  • 阳性对照
    • HepG2 cell extracts. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach

性能

应用

Our Abpromise guarantee covers the use of ab65242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1.
  • 组织特异性Widely expressed. Abundant expression seen in the pancreas and testis.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 azacytidine induced protein 2 antibody
    • 5-azacytidine-induced protein 2 antibody
    • AA410145 antibody
    • AZ2 antibody
    • AZI2 antibody
    • AZI2_HUMAN antibody
    • MGC112644 antibody
    • Nak associated protein 1 antibody
    • Nak-associated protein 1 antibody
    • NAP1 antibody
    • NF kappa B activating kinase associated protein 1 antibody
    • NF-kappa-B-activating kinase-associated protein 1 antibody
    • TILP antibody
    • TILP(S) antibody
    see all

Anti-AZI2 antibody 图像

  • IHC image of AZI2 staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65242, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of AZI2 staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abab65242, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-AZI2 antibody (ab65242) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide


    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa,70 kDa. We are unsure as to the identity of these extra bands.
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
  • ICC/IF image of ab65242 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65242, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-AZI2 antibody (ab65242)参考文献

ab65242 has not yet been referenced specifically in any publications.

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