概述

  • 产品名称Anti-AZI1抗体
    参阅全部 AZI1 一抗
  • 描述
    兔多克隆抗体to AZI1
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 1033 and 1083 of human AZI1 (EAW89632.1)

  • 阳性对照
    • HeLa Cells.
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified using an epitope specific to AZI1 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed or Acetone fixed cells.
    Permeabilization with Triton-X 100 is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May play a role in spermatogenesis.
    • 序列相似性Contains 1 IQ domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5 azacytidine induced 1 antibody
      • 5-azacytidine-induced protein 1 antibody
      • AZ1 antibody
      • Azi antibody
      • Azi1 antibody
      • AZI1_HUMAN antibody
      • Centrosomal protein 131 kDa antibody
      • Centrosomal protein of 131 kDa antibody
      • Cep131 antibody
      • KIAA1118 antibody
      • OTTMUSP00000004498 antibody
      • Pre-acrosome localization protein 1 antibody
      • RP23 37J21.1 antibody
      see all

    Anti-AZI1 antibody 图像

    • ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
    • ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

    Anti-AZI1 antibody (ab84864)参考文献

    This product has been referenced in:
    • Hoang-Minh LB  et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). ICC/IF ; Human . Read more (PubMed: 27661404) »
    • Tollenaere MA  et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun 6:10075 (2015). IP ; Human . Read more (PubMed: 26616734) »

    See all 4 Publications for this product

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS cells)
    Loading amount 50 µg
    Specification U2OS cells
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"