Anti-Axl抗体[MM0098-2N33] (ab89224)

概述

  • 产品名称Anti-Axl抗体[MM0098-2N33]
    参阅全部 Axl 一抗
  • 描述
    小鼠单克隆抗体[MM0098-2N33] to Axl
  • 经测试应用适用于: IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human, African Green Monkey
  • 免疫原

    Human recombinant Axl (extracellular region).

性能

应用

Our Abpromise guarantee covers the use of ab89224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody [MM0098-2N33] 图像

  • ab89224 used in Flow Cytometry.
    Cells were trypsinized for 1 minute at room temperature and wahed once in cold PBS. They were stained with ab89224 diluted 1/200 in 5% BSA/PBS for 45 minutes on ice, washed and incbuated with ab98794 at a 1/200 dilution. Cells were filtered and read in a FACScan using the FL1 sensor.
  • ab89224 staining Axl in african green monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde and then blocked using 5% serum for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 546 (red) used at a 1/400 dilution. DAPI was used to counterstain nuclei.

Anti-Axl antibody [MM0098-2N33] (ab89224)参考文献

ab89224 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that this product does not contain any azide. It only contains PBS.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (HeLa)
Specification HeLa
Preparation Cell harvesting/tissue preparation method: Cells were collected by trypsinization
Sample buffer: PBS
Fixation no fixation
Permeabilization No
Username

Mr. Nir Drayman

Verified customer

提交于 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample African Green Monkey Cell (CV1)
Specification CV1
Fixation no fixation
Permeabilization No
Username

Mr. Nir Drayman

Verified customer

提交于 May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (CV1 cells)
Total protein in input 1e+007 cells
Specification CV1 cells
Immuno-precipitation step Protein A/G
Username

Mr. Nir Drayman

Verified customer

提交于 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (CV1)
Specification CV1
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

提交于 Apr 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"