概述

  • 产品名称
    Anti-Axl抗体- C-terminal
    参阅全部 Axl 一抗
  • 描述
    兔多克隆抗体to Axl - C-terminal
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Axl aa 863-894 (C terminal) conjugated to keyhole limpet haemocyanin.

  • 阳性对照
    • SKBR3 cell lysate. Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab72069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).

靶标

  • 功能
    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性
    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody - C-terminal 图像

  • Anti-Axl antibody - C-terminal (ab72069) at 1/100 dilution + SKBR3 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 98 kDa
    Observed band size : 98 kDa
    Additional bands at : 25 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • ab72069 at 1/50 dilution, staining Axl in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-Axl antibody - C-terminal (ab72069)参考文献

This product has been referenced in:
  • Dine JL  et al. The TRAIL receptor agonist drozitumab targets basal B triple-negative breast cancer cells that express vimentin and Axl. Breast Cancer Res Treat 155:235-51 (2016). IHC ; Human . Read more (PubMed: 26759246) »
  • Hsieh MS  et al. The AXL receptor tyrosine kinase is associated with adverse prognosis and distant metastasis in esophageal squamous cell carcinoma. Oncotarget 7:36956-36970 (2016). WB, IHC . Read more (PubMed: 27172793) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MEFs)
Loading amount
10 µg
Specification
MEFs
Treatment
human Axl over expression
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

提交于 Jan 05 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1 cells)
Specification
CV1 cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

提交于 Jan 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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