概述

  • 产品名称Anti-Axl抗体
    参阅全部 Axl 一抗
  • 描述
    兔多克隆抗体to Axl
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cyt, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Axl aa 21-37 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    RGTQAEESPFVGNPGNI


    Database link: P30530

  • 阳性对照
    • WB: SKBR3 cell lysate. ICC/IF: SW480 cells. IHC-P: Human breast cancer tissue. Flow Cyt: WiDr cells.

性能

应用

Our Abpromise guarantee covers the use of ab37861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 24236135
WB 1/1000. Detects a band of approximately 97 kDa.

靶标

  • 功能May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody 图像

  • Anti-Axl antibody (ab37861) + SKBR3 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG
    Developed using the ECL technique

    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer (BC) tissue labelling Axl with ab37861. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

  • ab37861 staining Axl in human breast tumor by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween20 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with the primary antibody (in PBS + 10% serum) for 12 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated goat anti-rabbit IgG monoclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab37861 stained SW480 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37861 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow cytometry analysis of WiDr cells labelling Axl (green) with ab37861 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-Axl antibody (ab37861)参考文献

This product has been referenced in:
  • Zhu HY  et al. Peroxisome proliferator-activated receptor-? agonist troglitazone suppresses transforming growth factor-ß1 signalling through miR-92b upregulation-inhibited Axl expression in human keloid fibroblasts in vitro. Am J Transl Res 8:3460-70 (2016). WB ; Human . Read more (PubMed: 27648136) »
  • Lee CH  et al. Expression of growth arrest-specific protein 6 and Axl molecules in the left internal mammary artery of patients undergoing coronary artery bypass grafting. J Clin Pathol 67:506-11 (2014). IHC ; Human . Read more (PubMed: 24567414) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (Breast tumour)
Specification Breast tumour
Permeabilization Yes - Tween20
Fixative Formaldehyde
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Mr. Gabriel Ortega

Verified customer

提交于 Mar 24 2014

Thank you for calling Abcam earlier.

I am sorry about the issues you have been having with ab37861 in western blot. As we discussed, I am going to send you ab123597 as a free of charge replacement as soon as it comes back into stock. Your new...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"