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Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse Axin 2.
(Peptide available as ab32196.)
Our Abpromise guarantee covers the use of ab32197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Can be blocked with Mouse Axin 2 peptide (ab32196). Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.|
|Flow Cyt||1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
Flow Cytometry analysis of human permeabilised REH B cells labeling Axin 2 with ab32197 (red). Cells were fixed with paraformaldehyde and permeabilized with saponin, followed by incubation with 2ul of undiluted Anti-Axin 2 antibody (ab32197) in 100µl of cells in PBS for 15 minutes at 4°C. An undiluted polyclonal goat anti-rabbit Alexa Fluor® 488 was used as the secondary antibody. Control - rabbit IgG (white).
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