概述

  • 产品名称Anti-Axin 1抗体
    参阅全部 Axin 1 一抗
  • 描述
    小鼠单克隆抗体to Axin 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ISRHRRTGHG SSGTRKPQPH ENSRPLSLEH PWAGPQLRTS VQPSHLFIQD PTMPPHPAPN PLTQLEEARR RLEEEEKRAS RAPSKQRYVQ EVMRRGRA, corresponding to amino acids 643-741 of Human Axin 1

性能

应用

Our Abpromise guarantee covers the use of ab56475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
    • 组织特异性Ubiquitously expressed.
    • 疾病相关Hepatocellular carcinoma
      Caudal duplication anomaly
    • 序列相似性Contains 1 DIX domain.
      Contains 1 RGS domain.
    • 结构域The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
    • 翻译后修饰Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
      ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
      Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
    • 细胞定位Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI316800 antibody
      • AXIN antibody
      • Axin 1 antibody
      • Axin-1 antibody
      • axin1 antibody
      • AXIN1_HUMAN antibody
      • Axis inhibition protein 1 antibody
      • Axis Inhibitor 1 antibody
      • Fu antibody
      • fused antibody
      • Fused, mouse, homolog of antibody
      • hAxin antibody
      • Kb antibody
      • Ki antibody
      • kinky antibody
      • knobbly antibody
      • MGC132911 antibody
      • MGC52315 antibody
      • PPP1R49 antibody
      • Protein Fused antibody
      • Protein phosphatase 1 regulatory subunit 49 antibody
      see all

    Anti-Axin 1 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56475 Axin 1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-Axin 1 antibody (ab56475)参考文献

    ab56475 has not yet been referenced specifically in any publications.

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