Anti-Axin 1抗体(ab4157)


  • 产品名称
  • 描述
    山羊多克隆抗体to Axin 1
  • 宿主
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: FEEKIIGKVEKVD, corresponding to C terminal amino acids 850-862 of Human AXIN1.

  • 常规说明
    Principal Names - AXIN1; AXIN; MGC52315; axin 1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1 Official Gene Symbol - AXIN1 GenBank Accession Number – NP_003493; NP_851393 LocusLink ID - 8312 (human); 12005 (mouse); 79257 (rat) Gene Ontology terms - signal transducer activity; oocyte axis determination; frizzled receptor signaling pathway; development; apoptosis; cytoplasm; negative regulation of cell cycle.



Our Abpromise guarantee covers the use of ab4157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Can be blocked with Human Axin 1 peptide (ab23000). No signal obtained yet but low background observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2.0µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能
    Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • 组织特异性
    Ubiquitously expressed.
  • 疾病相关
    Hepatocellular carcinoma
    Caudal duplication anomaly
  • 序列相似性
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • 结构域
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • 翻译后修饰
    Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • 细胞定位
    Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all


  • All lanes : Anti-Axin 1 antibody (ab4157) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
    Lane 2 : Jurkat whole cell lysate (ab7899)

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat IgG secondary antibody (ab28445) at 1/10000 dilution
  • ab4157 (3.8 µg/ml) staining Azin 1 in Human adrenal cortex by IHC-P (Immunohistochemistry of paraffin-embedded sections). Steamed antigen retrievalwith citrate buffer (pH 6) was performed. Image shows membranous and fibrous staining in and around the secretory cells of the zona fasciculata in the cortex.


ab4157 has not yet been referenced specifically in any publications.


Thank you for youe email, I'm glad to hear that this antibody appears to be working for you. We value all feedback from researchers and since this is a Fast Track antibody, it is especially important. Once you have more information, please submit feedb...

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Thank you so much for your patience! :) As stated on the online datasheet, ab4157 is a "fast track" antibody and thus far, no signal has been obtained but low background was observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2...

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