Anti-Axin 1抗体(ab115205)

概述

  • 产品名称Anti-Axin 1抗体
    参阅全部 Axin 1 一抗
  • 描述
    山羊多克隆抗体to Axin 1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Zebrafish, Baboon, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    FEEKIIGKVEKVD

    (with a Cysteine residue linker), corresponding to C terminal amino acids 850-862 of Human Axin 1

  • 阳性对照
    • Human adrenal tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab115205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Hepatocellular carcinoma
    Caudal duplication anomaly
  • 序列相似性Contains 1 DIX domain.
    Contains 1 RGS domain.
  • 结构域The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • 翻译后修饰Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • 细胞定位Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all

Anti-Axin 1 antibody 图像

  • ab114205, at 3.75 ug/ml, staining Axin-1 in formalin-fixed, paraffin-embedded Human adrenal tissue by immunohistochemistry.
  • Immunohistochemical analysis of rat bladder tissue, staining Axin 1 with ab115205.

    Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

Anti-Axin 1 antibody (ab115205)参考文献

ab115205 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bladder)
Specification Bladder
Fixative 10% Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: .01M Citrate Buffer Ph 6.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
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提交于 Oct 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"