Anti-Avidin抗体(Biotin) (ab7235)

概述

  • 产品名称Anti-Avidin抗体(Biotin)
    参阅全部 Avidin 一抗
  • 描述
    兔多克隆抗体to Avidin (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • 免疫原

    Avidin [Hen Egg White]

  • 常规说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • 应用说明Immunoblotting, ELISA (1:8,000 to 1:32,000), immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
  • 靶标

    • 功能The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
    • 组织特异性Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
    • 序列相似性Belongs to the avidin/streptavidin family.
      Contains 1 avidin-like domain.
    • 翻译后修饰N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
    • 细胞定位Secreted.
    • Information by UniProt
    • 别名
      • AVD antibody
      • AVID_CHICK antibody
      • Avidin antibody
      • LOC396260 antibody

    Anti-Avidin antibody (Biotin) (ab7235)参考文献

    ab7235 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"