Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Avidin [Hen Egg White]. Cross reactivity against Avidin from other tissues may occur but have not been specifically determined.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/20,000 - 1/100,000.
IP: Use at an assay dependent dilution.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 17 kDa.
Suitable for most immunological methods requiring high titer and specificity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
Belongs to the avidin/streptavidin family. Contains 1 avidin-like domain.
N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.