Anti-Avian Influenza A Neuraminidase抗体(ab21304)


  • 产品名称Anti-Avian Influenza A Neuraminidase抗体
    参阅全部 Avian Influenza A Neuraminidase 一抗
  • 描述
    兔多克隆抗体to Avian Influenza A Neuraminidase
  • 经测试应用适用于: Flow Cyt, WB, ELISAmore details
  • 种属反应性
    Reacts with Avian Influenza A virus.
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids at the carboxy terminus of the Neuraminidase protein.



Our Abpromise guarantee covers the use of ab21304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 21151571ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. It will detect 10 ng of free peptide at 1 µg/ml.


  • 相关性Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
  • 细胞定位Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).
  • 别名
    • NA antibody
    • Neuraminidase antibody

Anti-Avian Influenza A Neuraminidase antibody 图像

  • Image from Hessel A et al, PLoS One. 2010 Aug 16;5(8). pii: e12217, Fig 1.

Anti-Avian Influenza A Neuraminidase antibody (ab21304)参考文献

This product has been referenced in:
  • Tripathi S  et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . Read more (PubMed: 23538443) »
  • Hessel A  et al. A pandemic influenza H1N1 live vaccine based on modified vaccinia Ankara is highly immunogenic and protects mice in active and passive immunizations. PLoS One 5: (2010). WB . Read more (PubMed: 20808939) »

See all 4 Publications for this product

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Thank you for your enquiry and your interest.

Tested applications are listed on the on-line product datasheets. Since IHC is not listed on neither of the documents (ab21304 and ab110599), this means that these two productsare not tested and c...

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Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Goat Recombinant protein (LMH cells)
Loading amount 10 µg
Specification LMH cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 02 2010

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 4...

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Thank you for your enquiry. A BLAST search on the sequence reveals that Ab21304 should cross-react with neuraminidase from the following strains: H1N1,H4N1, H6N1, H7N1, H9N1 and H11N1. If you have any further questions then please get back in contact w...

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Thank you for getting back to me. Once again, unfortunately I cannot comment on the peptide sequence that this antiserum was raised against as it remains proprietary. However, given the sequence alignment that you have performed it seems that there...

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Thank you for your enquiry. Unfortunately the immunogen for this antibody remains proprietary. The peptide sequence used to generate ab21304 is not from the very C-terminus, but from the C-terminal region. It has 13/15 (*******:*****.*) identical r...

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