重组Anti-Aurora B抗体[EP1009Y] (ab45145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1009Y] to Aurora B
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Aurora B抗体[EP1009Y]
参阅全部 Aurora B 一抗 -
描述
兔单克隆抗体[EP1009Y] to Aurora B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Aurora B aa 1-100 (N terminal). The exact sequence is proprietary.
-
阳性对照
- IP: HeLa cell lysate WB: HeLa cell lysate IHC: human endometrium carcinoma ICC: HeLa cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数(KD)
KD = 5.50 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1009Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/300.
|
|
ICC/IF |
1/20.
For unpurified version use 1/100 - 1/150 dilution |
|
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
For unpurified version use at 1/50000 dilution |
IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified version use at 1/250-500 |
|
IP |
Use at an assay dependent concentration.
For unpurified version use at 1/20 dilution |
说明 |
---|
Flow Cyt (Intra)
1/300. |
ICC/IF
1/20. For unpurified version use 1/100 - 1/150 dilution |
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. For unpurified version use at 1/50000 dilution |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified version use at 1/250-500 |
IP
Use at an assay dependent concentration. For unpurified version use at 1/20 dilution |
靶标
-
功能
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
组织特异性
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
疾病相关
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
-
数据库链接
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
-
别名
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
图片
-
Lanes 1-2 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/1000 dilution
Lanes 3-4 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/5000 dilution
Lanes 5-6 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/10000 dilution
Lanes 7-8 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/50000 dilution
Lanes 9-10 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/75000 dilution
Lanes 1 & 3 & 5 & 7 & 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 4 & 6 & 8 & 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Nocodozole Stimulated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45145 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
-
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium carcinoma tissue sections labeling Aurora B with purified ab45145 at 1/200 dilution (1.35 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1/300 dilution (0.1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabeled control - /.
-
Purified ab45145 at 1/20 dilution (1µg) immunoprecipitating Aurora B in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab45145 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45145 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (24)
ab45145 被引用在 24 文献中.
- Dong P et al. Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells. Exp Ther Med 23:164 (2022). PubMed: 35069845
- Liu M et al. Role of aurora kinase B in regulating resistance to paclitaxel in breast cancer cells. Hum Cell 35:678-693 (2022). PubMed: 35088239
- Li Q et al. CKAP2L, a crucial target of miR-326, promotes prostate cancer progression. BMC Cancer 22:666 (2022). PubMed: 35715760
- Liao L et al. Non-SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF-7 cells. Oncol Lett 24:317 (2022). PubMed: 35949592
- Tsunematsu T et al. APC/CCdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit. J Cell Sci 133:N/A (2020). PubMed: 32934012