Anti-Aurora A (phospho T288)抗体(ab83968)


  • 产品名称Anti-Aurora A (phospho T288)抗体
    参阅全部 Aurora A 一抗
  • 描述
    兔多克隆抗体to Aurora A (phospho T288)
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Aurora A (phospho T288).
    Database link: NP_003591.2

  • 阳性对照
    • NBF fixed asynchronous HeLa cells.



Our Abpromise guarantee covers the use of ab83968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.

Formaldehyde fixed cells. Permeabilisation with Triton-X100 is recommended.


  • 功能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 组织特异性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A (phospho T288) antibody 图像

  • ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
    Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100.
  • ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
    Mock phosphatase treated section (left), or calf intestinal phosphatase treated section (right).
    Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100.

Anti-Aurora A (phospho T288) antibody (ab83968)参考文献

This product has been referenced in:
  • Whalley HJ  et al. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun 6:7437 (2015). ICC/IF ; Human . Read more (PubMed: 26078008) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH 3T3)
Permeabilization No
Specification NIH 3T3
Fixative Methanol

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提交于 Apr 14 2015

Gracias a ti por tu amable respuesta.

No hay ningún problema, creo que lo menos que podíamos hacer para compensar por el error en la datasheet es reemplazaros los dos anticuerpos correspondientes.

Estos dos productos q...

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Thank you for contacting us yesterday.

I can confirm ab83968 has always been raised in rabbit and the vial you have received is from rabbit. We made a mistake on our datasheet and pig should not of been the selected host species. Therefore p...

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